检索范围:
排序: 展示方式:
null
《医学前沿(英文)》 2013年 第7卷 第4期 页码 462-476 doi: 10.1007/s11684-013-0270-6
Evaluating the effects of novel drugs on appropriate tumor models has become crucial for developing more effective therapies that target highly tumorigenic and drug-resistant cancer stem cell (CSC) populations. In this study, we demonstrate that a subset of cancer cells with CSC properties may be enriched into tumor spheroids under stem cell conditions from a non-small cell lung cancer cell line. Treating these CSC-like cells with gemcitabine alone and a combination of gemcitabine and the novel CHK1 inhibitor PF-00477736 revealed that PF-00477736 enhances the anti-proliferative effect of gemcitabine against both the parental and the CSC-like cell populations. However, the CSC-like cells exhibited resistance to gemcitabine-induced apoptosis. Collectively, the spheroid-forming CSC-like cells may serve as a model system for understanding the mechanism underlying the drug resistance of CSCs and for guiding the development of better therapies that can inhibit tumor growth and eradicate CSCs.
关键词: drug resistance cancer stem cell checkpoint kinase 1 (CHK1) PF-00477736 lung cancer tumorigenicity
Andrew Best,Katherine James,Gerald Hysenaj,Alison Tyson-Capper,David J. Elliott
《化学科学与工程前沿(英文)》 2016年 第10卷 第2期 页码 186-195 doi: 10.1007/s11705-015-1540-4
关键词: RNA splicing gene expression breast cancer DNA damage CHK1
Molecular classification and precision therapy of cancer: immune checkpoint inhibitors
null
《医学前沿(英文)》 2018年 第12卷 第2期 页码 229-235 doi: 10.1007/s11684-017-0581-0
On May 23, 2017, the US Food and Drug Administration (FDA) approved a treatment for cancer patients with positive microsatellite instability-high (MSI-H) markers or mismatch repair deficient (dMMR) markers. This approach is the first approved tumor treatment using a common biomarker rather than specified tumor locations in the body. FDA previously approved Keytruda for treatment of several types of malignancies, such as metastatic melanoma, metastatic non-small-cell lung cancer, recurrent or metastatic head and neck cancer, refractory Hodgkin lymphoma, and urothelial carcinoma, all of which carry positive programmed death-1/programmed death-ligand 1 biomarkers. Therefore, indications of Keytruda significantly expanded. Several types of malignancies are disclosed by MSI-H status due to dMMR and characterized by increased neoantigen load, which elicits intense host immune response in tumor microenvironment, including portions of colorectal and gastric carcinomas. Currently, biomarker-based patient selection remains a challenge. Pathologists play important roles in evaluating histology and biomarker results and establishing detection methods. Taking gastric cancer as an example, its molecular classification is built on genome abnormalities, but it lacks acceptable clinical characteristics. Pathologists are expected to act as “genetic interpreters” or “genetic translators” and build a link between molecular subtypes with tumor histological features. Subsequently, by using their findings, oncologists will carry out targeted therapy based on molecular classification.
关键词: molecular classification precision medicine pembrolizumab PD-1/PD-L1 MSI-H
《医学前沿(英文)》 页码 805-822 doi: 10.1007/s11684-023-1025-7
关键词: CTLA-4 PD-1 PD-L1 immune checkpoint blockade (ICB) metabolic reprogramming combined tumor therapeutic strategies
Activation of phagocytosis by immune checkpoint blockade
null
《医学前沿(英文)》 2018年 第12卷 第4期 页码 473-480 doi: 10.1007/s11684-018-0657-5
Inhibition of macrophage-mediated phagocytosis has emerged as an essential mechanism for tumor immune evasion. One mechanism inhibiting the innate response is the presence of the macrophage inhibitory molecule, signal regulatory protein-α (SIRPα), on tumor-associated macrophages (TAMs) and its cognate ligand cluster of differentiation 47 (CD47) on tumor cells in the tumor microenvironment. On the basis of a recently discovered programmed death protein 1 (PD-1) in TAMs, we discuss the potential inhibitory receptors that possess new functions beyond T cell exhaustion in this review. As more and more immune receptors are found to be expressed on TAMs, the corresponding therapies may also stimulate macrophages for phagocytosis and thereby provide extra anti-tumor benefits in cancer therapy. Therefore, identification of biomarkers and combinatorial therapeutic strategies, have the potential to improve the efficacy and safety profiles of current immunotherapies.
关键词: CD47 PD-1 PD-L1 immunotherapy TAM phagocytosis macrophage
PD-1/PD-L1 blockade in cervical cancer: current studies and perspectives
Yumeng Wang, Guiling Li
《医学前沿(英文)》 2019年 第13卷 第4期 页码 438-450 doi: 10.1007/s11684-018-0674-4
关键词: PD-1 PD-L1 immune checkpoint blockade antibody immunotherapy cervical cancer
Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice
null
《医学前沿(英文)》 2016年 第10卷 第4期 页码 481-489 doi: 10.1007/s11684-016-0494-3
The tumor suppressor gene liver kinase B1 (LKB1), also called STK11, encodes a serine/threonine kinase. LKB1 plays crucial roles in cell differentiation, proliferation, and polarity. In this study, LKB1 conditional knockout mice (LKB1Pax2 CKO mice) were generated using Pax2-Cre mice to investigate the function of LKB1 in inner ear hair cells during early embryonic period. LKB1Pax2 CKO mice died perinatally. Immunofluorescence and scanning electron microscopy revealed that stereociliary bundles in LKB1Pax2 CKO mice were clustered and misoriented, respectively. Moreover, ectopic distribution of kinocilium bundles resulting from abnormal migration of kinocilium was observed in the mutant mice. The orientation of stereociliary bundles and the migration of kinocilia are critical indicators of planar cell polarity (PCP) of hair cells. LKB1 deficiency in LKB1Pax2 CKO mice thus disrupted hair cell planar polarity during embryonic development. Our results suggest that LKB1 is required in PCP formation in cochlear hair cells in mice.
关键词: LKB1 stereociliary bundles kinocilium planar cell polarity hearing mice
Monitoring checkpoint inhibitors: predictive biomarkers in immunotherapy
Min Zhang, Jingwen Yang, Wenjing Hua, Zhong Li, Zenghui Xu, Qijun Qian
《医学前沿(英文)》 2019年 第13卷 第1期 页码 32-44 doi: 10.1007/s11684-018-0678-0
Immunotherapy has become the fourth cancer therapy after surgery, chemotherapy, and radiotherapy. In particular, immune checkpoint inhibitors are proved to be unprecedentedly in increasing the overall survival rates of patients with refractory cancers, such as advanced melanoma, non-small cell lung cancer, and renal cell carcinoma. However, inhibitor therapies are only effective in a small proportion of patients with problems, such as side effects and high costs. Therefore, doctors urgently need reliable predictive biomarkers for checkpoint inhibitor therapies to choose the optimal therapies. Here, we review the biomarkers that can serve as potential predictors of the outcomes of immune checkpoint inhibitor treatment, including tumor-specific profiles and tumor microenvironment evaluation and other factors.
关键词: immune checkpoint companion diagnosis PD-L1 tumor mutation burden immune score
PAK1 is a novel cardiac protective signaling molecule
null
《医学前沿(英文)》 2014年 第8卷 第4期 页码 399-403 doi: 10.1007/s11684-014-0380-9
We review here the novel cardiac protective effects of the multifunctional enzyme, p21-activated kinase 1 (PAK1), a member of a serine/threonine protein kinase family. Despite the large body of evidence from studies in noncardiac tissue indicating that PAK1 activity is key in the regulation of a number of cellular functions, the role of PAK1 in the heart has only been revealed over the past few years. In this review, we assemble an overview of the recent findings on PAK1 signaling in the heart, particularly its cardiac protective effects. We present a model for PAK1 signaling that provides a mechanism for specifically affecting cardiac cellular processes in which regulation of protein phosphorylation states by protein phosphatase 2A (PP2A) predominates. We discuss the anti-adrenergic and antihypertrophic cardiac protective effects of PAK1, as well as its role in maintaining ventricular Ca2+ homeostasis and electrophysiological stability under physiological, β-adrenergic and hypertrophic stress conditions.
《医学前沿(英文)》 页码 878-888 doi: 10.1007/s11684-023-0993-y
关键词: uveal melanoma liver-directed therapy immune checkpoint blockade SIRT anti-PD-1 anti-CTLA-4
The role of CDK1 siRNA interference in cell cycle and cell apoptosis
Hui XIAO PhD, Ming TIAN MM, Junna GE MM, Xin Wei MD, Zhaoming LI MM, Xiaolan LI MS, Deding TAO PhD, Junbo HU MD, Jianping GONG MD,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 384-389 doi: 10.1007/s11684-009-0070-1
关键词: cyclin-dependent kinase1 siRNA interference cell cycle apoptosis
灰色预测模型GM(1,1)的适用性分析及在火灾风险预测中的应用
陈子锦,王福亮,陆守香
《中国工程科学》 2007年 第9卷 第5期 页码 91-94
通过对灰色预测模型———GM(1,1)的理论分析,证明了该模型的预测值及其变化趋势均具有单调 性,进而提出了GM(1,1)模型的适用性判据,并给出了该判据在火灾风险灰色预测中的应用实例。
邓运华
《中国工程科学》 2011年 第13卷 第10期 页码 13-18
联邦无监督表示学习 Research Article
张凤达1,况琨1,陈隆1,游兆阳1,沈弢1,肖俊1,张寅1,吴超2,吴飞1,庄越挺1,李晓林3,4,5
《信息与电子工程前沿(英文)》 2023年 第24卷 第8期 页码 1181-1193 doi: 10.1631/FITEE.2200268
关键词: 联邦学习;无监督学习;表示学习;对比学习
王正新,党耀国,刘思峰
《中国工程科学》 2012年 第14卷 第7期 页码 98-102
针对工程中大量存在的非等间距序列的建模问题,提出了非等间距GM(1, 1)幂模型。以平均相对误差绝对值最小化为目标,以模型参数之间的关系为约束,构建了一个非线性优化模型实现非等间距GM(1, 1)幂模型的参数估计。结果表明,非等间距GM(1, 1)幂模型的形式较为灵活,非等间距GM(1, 1)模型和灰色Verhulst模型均是非等间距GM(1, 1)幂模型的特殊情形,幂指数的优化有利于提高建模精度。最后通过一个工程实例验证了非等间距GM(1, 1)幂模型的有效性与实用性。
标题 作者 时间 类型 操作
Combined gemcitabine and CHK1 inhibitor treatment induces apoptosis resistance in cancer stem cell-like
null
期刊论文
protect breast cancer cells from accumulating replication stress by ensuring productive splicing of checkpointkinase 1
Andrew Best,Katherine James,Gerald Hysenaj,Alison Tyson-Capper,David J. Elliott
期刊论文
Metabolic interventions combined with CTLA-4 and PD-1/PD-L1 blockade for the treatment of tumors: mechanisms
期刊论文
PD-1/PD-L1 blockade in cervical cancer: current studies and perspectives
Yumeng Wang, Guiling Li
期刊论文
Monitoring checkpoint inhibitors: predictive biomarkers in immunotherapy
Min Zhang, Jingwen Yang, Wenjing Hua, Zhong Li, Zenghui Xu, Qijun Qian
期刊论文
Liver-directed treatment is associated with improved survival and increased response to immune checkpoint
期刊论文
The role of CDK1 siRNA interference in cell cycle and cell apoptosis
Hui XIAO PhD, Ming TIAN MM, Junna GE MM, Xin Wei MD, Zhaoming LI MM, Xiaolan LI MS, Deding TAO PhD, Junbo HU MD, Jianping GONG MD,
期刊论文